The virulence test of five species of entomogenous fungi Beauveria brongniartii, Beauveria bassiana, Paecilomyces fumosoroseus, Metarhizium anisopliae and Nomuraea rileyi to Spodoptera litura larvae showed that B. brongniartii and N. rileyi had evident pathogenic effects on S. litura, with the LT50 value to S. litura 2nd instars being 2.95 and 4.10 days, and the corrected accumulative mortality of the instars being 100% and 95.2%, respectively. The virulence of B. brongniartii and N. rileyi to the 3rd instars was lower than that of 2nd instars. The LT50 value to 3rd instars was 19.67 and 19.63 days, and the corrected accumulative mortality was 56.6% and 52.2%, respectively. Other two fungal species P. fumosoroseus and B. bassiana also had virulence to S. litura larvae. The LT50 value for the 2nd instars was 4.89 and 6.34 days, and the corrected accumulative mortality reached 85.7% and 71.4%, respectively.