[Purification and analysis of Cimicifuga foetida glycoprotein ( CF- I )].

Yu-jun, Sun; Yan, Chen; Zhi-yin, Song; Dong-wen, Zhou

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Article . 2007

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[Purification and analysis of Cimicifuga foetida glycoprotein ( CF- I )].

descriptionPublicationkeyboard_double_arrow_right Article 14 Sep 2007Journal:Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, volume 30 (issn: 1001-4454,

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Authors: Yu-jun, Sun; Yan, Chen; Zhi-yin, Song; Dong-wen, Zhou;

pmid: 17571759

[Purification and analysis of Cimicifuga foetida glycoprotein ( CF- I )].

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Abstract

A kind of glycoprotein ( CF- I ) was extracted from Cimicifuga foetida and purified by DEAE-Cellulose ( DEAE-52) and Sepharose CL-4B. It was identified to be homogeneous glycoprotein complex by electrophoresis and fast protein liquid chromatography (FPLC). alpha-glucosidic bond was detrermined by IR. Typical absorption of polysaccharide was shown in its IR spectrum. It had no typical absorption of nucleic acid or pigment by UV scanning. Glucose, galactose, mannose and arabinose were identified in CF- I with the molar ratio of 11. 94: 2. 18: 1. 38: 1 by GC. Its average MW was estimated to be 5. 8 x 10' by gel filtration. The content of total saccharide, protein and acid polysaccharide was 78% , 14. 4% and 23% respectively. It might be composed of 1-->4 and 1-->6 linked glucopyranose by periodate oxidation and Smith degration.

Related Organizations

Anhui University
China (People's Republic of)

Keywords

Cimicifuga, Hot Temperature, Plants, Medicinal, Molecular Structure, Galactose, Molecular Weight, Glucose, Uronic Acids, Spectrophotometry, Ultraviolet, Chromatography, High Pressure Liquid, Rhizome, Glycoproteins

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