????????: ?????????????? ?????????????? ???????????????????? ???? ?????????????? ???????????????????? ???????????????????? ?? ?????????????????????????? ???????????????? ?? ???????????????????????? ???????????????? ???????????????????? ?????????????????????? ?? ?????? (MDA-DNA) ?????? ???????????????? ???????????????????????????? ?? ???????? ?????????? ??????????. ????????????: ???????????????????????? ???????????????????????????? ???????????????? ???????????????????? ???????????????????? ?????????????????? ?? ?????????????????? ?????????? ?? ?????????????????? ?????????????????????????????????????????? ?????????????????????? MDA-DNA ?? ?????????? ???????????? ????????. ????????????????????: ????????????????, ?????? ?????? ???????????????? ?????????????????????????????? ?????????????????????????????? ?? ?????????????????? ?????????? ???????????????? ?????????????????????? ?????????????????????????? ???????????????????? ??????????-????????????????????????, ???????????????????????????????????????? ????????????????, ???????????????????? ????????????????- ?? ????????????????????????????????????????????, ???????????????? ?? ???????????? ??????????????????, ??????????????????????????????????????, ???????????????????????????????????????????????? ?? 5-????????????????????????. ?????? ???????????????????? ???????????????????????????????????????????? ???????????????????????? ???????????????? ???????????????????? ?????????????????????? ???????????????? MDA-DNA ?? ?????????? ???????????? ???????? ???? ?????????????????????????????? ??????????????????????????????. ?????? ???????????????? ???????????????????? ?????????????????????? ???????????????? ?????????????? ?????????????????? ?????????????????? ?? ?????????????????? ?????????? ?? ???????????????????????? ???????????????? MDA-DNA ?? ?????????? ????????????. ????????????????????: ???????????????????? ???????????????????? ?????????? ???????? ???????????????????? ?????? ???????????????????????????? ???????????????? ?????????????????????????????? ??????????????????????????????, ???????????????????????????? N-?????????????????????????????????????? ?? ????????, ?? ???????? ???????????????? ?????????? ???????? ???????????????????????????? ???????????????????????????????????????? ???????????????????? ?????? ?????????????? ???????? ????????????.

Aim: To study the effect of silymarin on the levels of tumor markers and MDA (malondialdehyde) ??? DNA adduct formation during N-nitrosodiethylamine induced hepatocellular carcinoma in male Wistar albino rats. Methods: The levels of AFP, CEA and activities of liver marker enzymes in serum, MDA-DNA immunohistochemistry were done according to standard procedures in the control and experimental groups of rats. Results: Hepatocellular carcinoma was evidenced from significant (p < 0.05) increases of alpha-fetoprotein, carcinoembryonic antigen, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, gamma-glutamyltransferase and 5?-nucleotidase in serum and increased MDA-DNA adducts were also observed in the tissue sections of hepatocellular carcinoma. Silymarin treatment significantly attenuated the alteration of these markers and decreased the levels of MDA-DNA adduct formation. Conclusion: Silymarin could be developed as a promising chemotherapeutic adjuvant for the treatment of liver cancer.