In SLO microperimetry a multi-channel overlay graphics frame grabber synchronizes the scanning of an infra-red laser with the modulation of a visible laser source. Graphics are created in the laser raster with a fast 25 ns sub-pixel rise-time 80 MHz acousto-optic modulator, Bragg angle optimized for 532 nm, 543.5 nm, 632.8 nm or 660 nm wavelengths over a 40 dB attenuation range. Our software kernel comprises 4 alternative forced choice (4AFC), parameter estimation by sequential testing (PEST) and manual or automated tracking algorithms based on two-dimensional normalized gray-scale correlation. They enable fast and accurate registration of fixation patterns, precise measurements of potential acuities and retinal sensitivity using a range of background illumination levels. Opto-electronic characteristics of physiologic significance are discussed. Clinical examples are given.