
The efficiency of the cleavage of RNA involved in perfect as well as imperfect hybrid duplexes composed of three components: (1) homogeneous RNA's or polyribonucleotides; (2) corresponding complementary synthetic oligodeoxyribonucleotides; (3) E. coli RNase H was investigated. The predominant RNA hydrolysis was shown to take place within the perfect hybrid duplexes formed by the target RNA and the complementary oligodeoxyribonucleotide probes. RNase H was found to cleave effectively a number of imperfect hybrid duplexes containing a central base pair mismatch.
Base Sequence, Hydrolysis, Molecular Sequence Data, Ribonuclease H, Escherichia coli, Autoradiography, Nucleic Acid Conformation, RNA, Electrophoresis, Polyacrylamide Gel, Oligonucleotide Probes, Catalysis
Base Sequence, Hydrolysis, Molecular Sequence Data, Ribonuclease H, Escherichia coli, Autoradiography, Nucleic Acid Conformation, RNA, Electrophoresis, Polyacrylamide Gel, Oligonucleotide Probes, Catalysis
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