A study was conducted to see the bacteriological profile in 172 cases of chronic dacryocystitis attending eye outpatients department of Midnapore Medical College and Hospital and NRS Medical College and Hospital, Kolkata. The patients underwent clinical examination of the eyes and lacrimal system, with particular attention to drainage system. The culture and sensitivity test was done from the material, which regurgitated through the punctum following pressure on sac. Out of 172 cases, 66(38.4%) were found with epiphora, 44 cases (25.9%) of epiphora with mass, 8 cases (4.6%) with epiphora mass with cellulitis and in 54 (31.4%) with chronic conjunctivitis. Bactriologically, 142 patients (82.5%) were revealed with pure culture, mixed culture in 18 cases (10.5%) and no growth of organism in 12 cases (7.0%). Staphylococcus aureus was found primary pathogen in disease process. Candida albicans were seen in 4 cases (2.3%).