The aim of this study was to investigate the different markers of stress in nail industry. Blood and urine biological samples were collected from 30 exposed workers and 30 age and sex matched control subjects from the administrative department of the same factory. The total antioxidant activity (Dmax) and lipid peroxidation was analyzed in blood. The level of catecholamines in the urine was also determined The median level of Dmaxwas significantly lower in the exposed workers (87.12) compared with that of the control (115.85) (p < 0.01). Also the level of lipid peroxidation was significantly lower in the exposed workers (44.5) compared with that of the control (74.0) (p < 0.01). The median level of urinary catecholamines was significantly higher in the exposed workers (295) compared with that of controls (37.5). Attention should be paid to the necessity of the application of the different biological markers of stress in different sectors of industry especially in nail industry.