As it becomes more and more difficult to find "easy" oil, various alternative fuels are introduced to the markets. These fuels have chemical properties that are different from the traditional gasoline and diesel fuels so that engine efficiency and other engine behaviors may be affected To improve engine efficiency and to identify which alternative fuel is the cleanest fuel solution, it is necessary to compile information about the ignition delay, which governs auto-ignition in spark-ignition (SI), compression-ignition (CI) and homogeneous charge compression-ignition (HCCI) engines. In this study, we measured ignition delay on the Rapid Compression Machine (RCM). RCM is a single-stroke device, which compresses uniform mixtures to engine-like condition. We can interpret from the pressure the detailed heat release process. A comprehensive ignition delay database of toluene/n-heptane mixtures and gasoline/ethanol mixtures was established The data allow us to calculate the auto-ignition behavior in engines. Depending on application the correct choice of alternative fuels may be made.