To set up a simple and effective oxygen-induced retinopathy (OIR) mouse model.Eyes from P17 mice in modified OIR group(Group A)and traditional OIR group (Group B) were snap frozen for immunohistochemical analysis with antibodies against GSL Flat-mounted, fluorescein-conjugated dextran-perfused retinas were examined to assess the retinal vasculature. The mortality of nursing mothers, survival rate of modeling mice and neovascularization ratio in Group A were obtained and compared with those in Group B.Loss of the central retinal vasculature and the neovascular tufts were observed in GSL retinal immunohistochemical analysis. A vascular area and neovasculature area were significantly larger and more typical in Group A than those in Group B. A lower mortality of nursing mothers, higher survival rate and neovascularization ratio of modeling mice was achieved in Group A.This modified oxygen-induced retinopathy mouse model is a simple, effective method, with more typical neovasculature were obtained.