[Determination of apoprotein A-1 by immunoenzyme analysis].
Copyright policy )[Determination of apoprotein A-1 by immunoenzyme analysis].
Indirect solid-phase immunoenzymatic assay was improved and adapted for quantitative estimation of apo A-I in blood serum. Immunochemical identification of apo A-I was carried out and specific antibodies were obtained. Various procedures of blood serum pretreatment affected dissimilarly the detection of antigenic determinants in apo A-I. Presence of tetramethyl urea in blood serum increased by 30% the latent antigenic determinants accessibility.
Immunoenzyme Techniques, Epitopes, Immunodiffusion, Apolipoprotein A-I, Blotting, Western, Humans, Electrophoresis, Polyacrylamide Gel, Antibodies, Apolipoproteins A
Immunoenzyme Techniques, Epitopes, Immunodiffusion, Apolipoprotein A-I, Blotting, Western, Humans, Electrophoresis, Polyacrylamide Gel, Antibodies, Apolipoproteins A
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