Fish proteins effect compared with casein was determined on triacylglycerols (TG) metabolism and activities of hepatic triacylglycerol lipase (HTGL) and tissue lipoprotein lipases (LPL), in SHR and diabetic SHR. Two groups of rats (n=24) were fed, 2 months, diet containing 20% casein (CAS) or 20% fish proteins (FP). One month before sacrifice, diabetes was induced in one group of rats by a single intraperitoneal injection of streptozotocin (STZ) (60 mg/kg of body weight). FP vs. CAS showed a significant decrease of TG concentrations in plasma (-41%) and liver (-66%), in SHR-STZ. VLDL-LDL mass, which represented the amount of apolipoproteins, TG, phospholipids (PL), unesterified cholesterol (UC), and cholesteryl esters (CE), decreased by 21 and 16% with FP vs. CAS, in SHR and SHR-STZ, respectively, and was concomitant with its low TG. Indeed, TG values was 1.35- and 1.82-fold lower in SHR and SHR-STZ, respectively. In HDL2, a TG diminution of 13% was noted, in SHR with FP vs. CAS. In SHR-STZ with FP, TG and PL were enhanced by 11% and 27%, respectively compared to SHR. HTGL activity decreased by 22% in SHR fed FP compared to those fed CAS. In SHR-STZ with CAS vsSHR, this activity was decreased by 21%. LPL activity in heart was enhanced by 19% in SHR-STZ vs. SHR with FP, when that of muscle was diminished 1.5-fold in SHR with FP vs. CAS and 1.8-fold in SHR-STZ vs. SHR with CAS. Adipose LPL activity was 1.36-fold higher in SHR with FP than CAS. In conclusion, it appears that fish proteins have a hypotriglyceridemic effect, which the mechanism can differ in SHR or SHR-STZ. It may be of interest to propose these fish proteins as lipid metabolism regulator in diseases with hypertriglyceridemia.