Canola (Brassica napus L.).
Copyright policy )Canola (Brassica napus L.).
High-frequency Agrobacterium tumefaciens-mediated transformation can be obtained in canola by optimizing the preconditioning time of the explant and cocultivation time with A. tumefaciens. A preconditioning time of 72 h and cocultivation of 48 h synergistically increase the transformation efficiency to 25%. In addition, the recovery of transgenic plants can be facilitated by overcoming hyperhydration, which increases the rooting frequency to 100%.
- University of Vermont United States
Time Factors, Transformation, Genetic, Agrobacterium tumefaciens, Brassica napus, Gene Transfer Techniques, Plants, Genetically Modified, Coculture Techniques
Time Factors, Transformation, Genetic, Agrobacterium tumefaciens, Brassica napus, Gene Transfer Techniques, Plants, Genetically Modified, Coculture Techniques
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