[Isolation and detection of murine transplantable breast tumor antigen by McAb F36/22].

G, Cai

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Article . 1990

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[Isolation and detection of murine transplantable breast tumor antigen by McAb F36/22].

descriptionPublicationkeyboard_double_arrow_right Article 13 Aug 1990Journal:Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao, volume 21 (issn: 0257-7712,

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Authors: G, Cai;

pmid: 1694814

[Isolation and detection of murine transplantable breast tumor antigen by McAb F36/22].

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Abstract

A transplantable murine breast tumor antigen (called JC tumor) has been recognized by the detection of McAb F36/22 with the method of immunoblotting. JC tumor antigen was isolated by Sepharose-CL-4B, then was purified by McAb F36/22 affinity chromatography. Solid phase RIA detection demonstrated that the specific activity of the antigen which was purified by 188.4 times was 148,000 cpm/mg protein. SDS-PAGE and immunoblot analysis revealed that the tumor antigen possessed a molecular weight of 60kd. Competitive binding RIA indicated that JC tumor antigen inhibited the combining of ductal carcinoma antigen with McAb F36/22. It implied that some groups (antigen determinant) on JC tumor antigen were the same as or similar to those on ductal cancer antigen.

Keywords

Epitopes, Mice, Mice, Inbred BALB C, Antigens, Neoplasm, Immunoblotting, Radioimmunoassay, Animals, Antibodies, Monoclonal, Mammary Neoplasms, Experimental, Chromatography, Affinity

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