A method of a one-row everted intestinal suture for the formation of terminal-terminal colon-colonic anastomosis was worked out in experiment in 32 dogs. The suture gives a good connection of fragments of the gut by wound surfaces by the type of sensory adaptation. High mechanical strength of the suture, absence of anastomosis stenosis, primary intension healing of the intestine wound were proved on the basis of using the method of pneumocompression, investigation of the stenosis index and histological investigation of the connection zone. The developed suture was used in 27 patients in forming anastomoses after operations on the colon. No complications resulting from the developed suture were noted.