[Study on tissue culture and rapid propagation of Atractylodes macrocephala].
Copyright policy )[Study on tissue culture and rapid propagation of Atractylodes macrocephala].
A study was conducted by tissue culture on the explant of Atractylodes macrocephala Koidz.. The result showed that NAA was a leading factor for induction of callus from leaf blades and the petiole as well as bud differentiation. MS + BA 1.0 mg/L + NAA 0.3 mg/L + GA3 0.2 mg/L was an optimal medium for induction of callus from leaf blades and the petiole and for the bud differentation also. MS + BA 3.0 mg/L + IBA 0.5 mg/L was suitable for the proliferation of axillary buds, with an induction rate of 95%. 1/2MS + IBA 0.1 - 0.5 mg/L was optimum for rooting, with a rooting percentage more than 90%. The survival rate of transplanted plantlets was more than 90%.
- Zhejiang Ocean University China (People's Republic of)
Plant Leaves, Tissue Culture Techniques, Plants, Medicinal, Plant Growth Regulators, Plant Stems, Atractylodes, Plant Shoots, Culture Media
Plant Leaves, Tissue Culture Techniques, Plants, Medicinal, Plant Growth Regulators, Plant Stems, Atractylodes, Plant Shoots, Culture Media
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