This paper aimed at improving existing breeds of Phellodendron chinense and enriching the resources of heredity breeding.The polyploid of P. chinense was obtained using calli, seeds and buds, which were treated with colchicines solution.The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%. Compared with the morphology and chromosomal number the polyploid induction was proved to be effective.It was an effective biotechnological way to use colchicines to induce polyploid on the different explants of P. chinense.