[Combined enzymatic modification of stevioside and rebaudioside A].

V T, Kochikian; A A, Markosian; L A, Abelian; A M, Balaian; V A, Abelian

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Прикладная биохимия и микробиология

Article . 2006

Data sources: Europe PubMed Central

[Combined enzymatic modification of stevioside and rebaudioside A].

descriptionPublicationkeyboard_double_arrow_right Article 14 Apr 2006 Russian Journal:Prikladnaia biokhimiia i mikrobiologiia, volume 42 (issn: 0555-1099,

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Authors: V T, Kochikian; A A, Markosian; L A, Abelian; A M, Balaian; V A, Abelian;

pmid: 16521574

[Combined enzymatic modification of stevioside and rebaudioside A].

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Abstract

Cyclodextrin glucanotransferases (CGTases, EC 2.4.1.19) produced by mesophilic, thermophilic, alkaliphilic, and halophilic bacilli were used for transglycosylating stevioside and rebaudiosides A with the use of starch as a donor. CGTases produced by B. stearothermophilus B-5076 B. macerans BIO-4m were the most effective biocatalysts. This method can be successfully used for direct transglycosylation of stevia extract without purification of its individual components.

Keywords

Glycosylation, Glucosides, Glucosyltransferases, Sweetening Agents, Stevia, Bacillus, Starch, Diterpenes, Kaurane, Chromatography, Liquid

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