
An essential first step in the purification of monoclonal antibodies is the clarification of the ascites fluid. While not ignored, this step has not received critical attention. Six different procedures were evaluated with respect to effectiveness and impact on several selected chemical components. For large-scale work, we find an on-line filtration process to be most efficient.
Mice, Inbred BALB C, Hybridomas, Antibodies, Monoclonal, Centrifugation, Silicon Dioxide, Mice, Animals, Ascitic Fluid, Caprylates, Filtration, Chlorofluorocarbons, Methane
Mice, Inbred BALB C, Hybridomas, Antibodies, Monoclonal, Centrifugation, Silicon Dioxide, Mice, Animals, Ascitic Fluid, Caprylates, Filtration, Chlorofluorocarbons, Methane
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