One hundred and ninety-one F2 individuals derives from the cross, Mo17xHuangzao4, were genotyped by SSR and AFLP markers to construct the genetic linkage map, and 184 corresponding F2:3 families were phenotyped for maize (Zea mays L.) plant hright and ear height in Changping and Shunyi. A mixed linear model approach and its software were used to detect QTLs with main effect, QTLs involved in digenic interations and Q x E interations. In total, 7 QTLs of plant height, 18 pairs of digenic epistatic loci of plant height, 13 pairs of digenic epistatic loci of ear height were detected. It was found that 6 QTLs of plant height, 8 QTLs of ear height, and 4 pairs of digenic epistatic loci of plant height and ear height and a significant interaction with environments. Genetic compnents underlying these quantitative traits were analyzed, and the study showed that additive, dominance and epistaship between plant height and ear height, and the results described above were also discussed for their possible application in molecular breeding.