A radioisotopic assay for proline oxidase activity.
Copyright policy )A radioisotopic assay for proline oxidase activity.
We developed a radioisotopic assay for proline oxidase in which product deltal-pyrroline-5-carboxylate-14-C is reacted with o-aminobenzaldehyde and the radioactivity trapped as the dihydroquinazolinium compound is recovered by ion-exchange chromatography. The sensitivity of this method allows the measurement of proline oxidase activity in small specimens (10 to 20 mg.) of liver.
Male, Proline, Cytochrome c Group, Chromatography, Ion Exchange, Rats, Liver, Benzaldehydes, Methods, Quinazolines, Animals, Colorimetry, Carbon Radioisotopes, Horses, Amines, Oxidoreductases
Male, Proline, Cytochrome c Group, Chromatography, Ion Exchange, Rats, Liver, Benzaldehydes, Methods, Quinazolines, Animals, Colorimetry, Carbon Radioisotopes, Horses, Amines, Oxidoreductases
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