To study the diagnostic value of combined detection of the 3 tumor markers, namely tissue polypeptide specific antigen (TPS), cancer antigen 153 (CA153), and carcino embryonic antigen (CEA), in patients with breast cancer.The serum levels of TPS, CA153 and CEA were determined by enzyme-linked immumosorbent assay in 90 patients with breast cancer and 30 healthy adults.The serum levels of 3 tumor markers in breast cancer patients were significantly higher than those in the control group (P<0.01). The sensitivity of TPS to breast cancer was 87.7% with specificity of 90.0%. The sensitivity and specificity of CA153 to breast cancer was 55.6% and 93.3%, and those of CEA was 45.8% and 96.7%, respectively. The sensitivity of the 3 tumor markers in combination was 100% in breast cancer diagnosis.TPS, CA153 and CEA have diagnostic value in breast cancer and possess obvious complementarity, and their combined detection can significantly improve the sensitivity of breast cancer diagnosis.