To assess the amount of sound transmission loss in order to deeply understand the mechanism of middle ear sound transmission for ossicular reconstruction and for guidance of crimping between prosthesis and stapes on sound transmission in stapes surgery.In order to get a correcting magnetic field 2 temporal bone models was used first. Temporal bone models was developed using 17 human temporal bones from fresh cadavers. To measure the sound transmission properties in the middle ear, 15 temporal bone models were detected by scanning laser Doppler interferometry with acoustical stimulation and electromechanical stimulation. An algorithm was developed to simulate acoustical stimulation with electromechanical stimulation. A sound transmission loss within the incudostapedial joint was detected by electromechanical stimulation in 10 temporal bone models.The average transmission loss within the incudostapedial joint remained 3 dB over the frequencies detected.There was a sound transmission loss within the incudostapedial joint, which was a physiological and protection way.