To investigate the changes of the multifocal electroretinogram (mfERG) in amblyopic eyes. Methods The mfERG of 28 anisometropic amblyopic cases (28 eyes), 25 strabismic amblyopic cases (25 eyes), 14 isometropic amblyopic cases (28 eyes) and 54 normal control eyes were tested.The N1 wave and P1 wave amplitude densities of mfERG first order kernel and the P1 wave amplitude densities of second order kernel in amblyopic eyes were significantly attenuated in the central region of the visual field (P 0.05). There was no significant difference in latencies of mfERG between the amblyopic eyes and control eyes (P > 0.05). No significant difference of mfERG was found among the three types of amblyopic eyes. In the amblyopic eyes, there was no linear correlation between visual acuity eyes and various indexes of mfERG (P > 0.05). There was significant difference in mfERG between the amblyopic eyes and contralateral eyes (P 0.05).The X ganglion cells in the retina of amblyopic eyes are abnormal, but the visual information transmitting time in the retina was normal in the amblyopic eyes.