To study the diagnostic value of cytokeraetin 19 fragment (CYFRA21-1) and tumor supplied group factors (TSGF) in nasopharyngeal carcinoma (NPC).Serum levels of CYFRA21-1 and TSGF in 82 patients with NPC, 25 patients with benign nasopharyngeal disease and 50 normal controls were measured by electrochemiluminescence immuno-assay.The levels of CYFRA21-1 and TSGF in NPC group were remarkably higher than those in benign nasopharyngeal disease and normal control groups (P < 0.01). Sensitivities of CYFRA21-1 and TSGF were 48.8% and 57.3%, respectively. The sensitivity of combined detection of two tumor markers increased to 74.3% and the specificity was not decreased significantly.CYFRA21-1 and TSGF can serve as the serum tumor marker in clinical diagnosis of NPC. Combined detection of two kinds of serum tumor markers increases the detective positive rate.