Many scientifical published works have documented the presence of macro and micronourished of truffle coming from Umbria, Toscana, and Piemonte, while are few the studies that documented the analysis of these nourishes of the truffle coming from Molise. In our study has been evaluated the concentrations of macro-nourishes and oligoelements in Molisano Tuber aestivum truffle. The contents of macro-nourishes (proteins, carbohydrates, lipids, and water) and oligoelements (lead, cadmium, chromium, manganese, zinc, nickel, and copper) have been determined. The results have demonstrated an adequate concentration of macronutrients, in particularly water and oligoelements, the latest important in many metabolic reaction of the body. A pathological concentration of heavy metals has not been found.