
A cDNA library was constructed from eight-day-old worker heads of Apis cerana cerana. A probe derived from part of Apis cerana genomic mrjp3 segment was used to screen this library. A total of 120 positive clones of mrjps were screened out from the cDNA library with DIG-probe. The positive clones were amplified and sequenced with T3/T7 primer. 12 sequences were similar to Apis cerana india and Apis mellifera mrjp1 gene by BLAST analysis. The cDNA sequence analysis indicated that the homology was 93.78% between Apis mellifera mrjp1 and Apis cerana mrjp1 while the homology was 99.36% between Apis cerana cerana and Apis cerana india. This result confirmed in molecular level that Apis cerana cerana and Apis cerana india had a common ancestor since the relationship between Apis cerana and Apis mellifera was more far.
DNA, Complementary, Base Sequence, Molecular Sequence Data, Animals, Insect Proteins, Amino Acid Sequence, Sequence Analysis, DNA, Bees, Cloning, Molecular
DNA, Complementary, Base Sequence, Molecular Sequence Data, Animals, Insect Proteins, Amino Acid Sequence, Sequence Analysis, DNA, Bees, Cloning, Molecular
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