Enterobacteria repetitive intergenic consensus sequences-based PCR(ERIC-PCR) was used to generate DNA fingerprints for Listeria spp. We got the specific profiles with ERIC-PCR technique that enables to identify Listeria species and the L.monocytogenes strains of different stereotype, and the same stereotype of L. monocytogenes from different sources also could be identified. Moreover,the species-specific 1600 bp DNA fragment was obtained from the fingerprint of L. monocytogenes. The study indicates that ERIC-PCR technique can be used in the identification of Listeria species and strains and its further typing, which is simple and quickly.