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[False positive and false negative reaction results based on an amplification of nucleic acids in infection diagnoses. Reasons and implications].

Authors: Agnieszka, Kamińska; Julia, Dabrowska;

[False positive and false negative reaction results based on an amplification of nucleic acids in infection diagnoses. Reasons and implications].

Abstract

The paper discusses the most frequent causes of false positive and false negative results of reactions based on amplification of nucleic acids. All stages of the method are described beginning with sample collection up to the finishing of visualization of amplification product. The main aim objective of the publication is to draw doctors' attention to the necessity to interpret the result with criticism. We attempted also to demonstrate how to avoid false results.

Keywords

DNA, Bacterial, Reproducibility of Results, Infections, Polymerase Chain Reaction, Sensitivity and Specificity, RNA, Bacterial, DNA, Viral, Animals, Humans, RNA, Viral, False Positive Reactions, False Negative Reactions, Nucleic Acid Amplification Techniques

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
gold
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