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[Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp].

descriptionPublicationkeyboard_double_arrow_right Article 03 May 2005Journal:Revista iberoamericana de micologia, volume 18 (issn: 1130-1406, Copyright policy )
Authors: M E, Sansinforiano; A, Rabasco; M, Martínez-Trancón; J C, Parejo; M, Hermoso-de-Mendoza; J A, Padilla;

pmid: 15487909

[Optimization of the conditions for RAPD-PCR of Candida spp. and Cryptococcus spp].

Abstract

In this study, we determined the optimal RAPD amplification conditions to obtain genetic molecular markers for the rapid and accurate identification of Cryptococcus spp. and Candida spp. The following parameters are modified: template DNA, DNA polymerase, magnesium cloride and primer concentration; denaturation, annealing and extension time, temperature of annealing and thermal cycles. After the optimization, reliable and reproducible RAPD patterns are obtained.

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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