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Прикладная биохимия ...arrow_drop_down

Прикладная биохимия и микробиология

Article . 1992

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[BME 361 I--a new site-specific deoxyribonuclease type II from Bacillus megaterium 361].

descriptionPublicationkeyboard_double_arrow_right Article 29 Jun 1992 Russian Journal:Prikladnaia biokhimiia i mikrobiologiia, volume 28 (issn: 0555-1099,

Authors: L R, Lebedev; I S, Andreeva; G D, Serov; L Ia, Denisova; N M, Pustoshilova;

pmid: 1534407

[BME 361 I--a new site-specific deoxyribonuclease type II from Bacillus megaterium 361].

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Abstract

Bme 361 I, a new site-specific type II deoxyribonuclease, was purified from Bacillus megaterium 361 by chromatography on phosphocellulose P 11 and hydroxylapatite. The enzyme recognizes and cleaves the nucleotide sequence 5'-GG decreases CC-3' in double-strand DNA. Thus it is a true isoschizomer of deoxyribonucleases Hae III and BspR I.

Keywords

DNA, Bacterial, Deoxyribonucleases, Hydrolysis, DNA, Viral, Bacillus megaterium, Autoradiography, Electrophoresis, Polyacrylamide Gel, Bacteriophage lambda, Chromatography, Liquid, Plasmids, Substrate Specificity

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