Instability of PRV-1 mRNA: a factor to be considered in PRV-1 quantification for the diagnosis of polycythemia vera.
Copyright policy )Instability of PRV-1 mRNA: a factor to be considered in PRV-1 quantification for the diagnosis of polycythemia vera.
High expression of PRV-1 mRNA in granulocytes has been proposed as a new diagnostic marker for polycythemia vera. We used real-time reverse transcription polymerase chain reaction (RT-PCR) to measure the levels of PRV-1 mRNA, GAPDH mRNA and 18S rRNA in granulocytes obtained from blood samples processed 2, 24 and 48 hours after collection and observed a significant decrease of PRV-1 levels after 24 and 48 hours. The instability of PRV-1 mRNA may affect the diagnostic value of the PRV-1 test in blood samples stored for extended periods.
Isoantigens, Membrane Glycoproteins, RNA Stability, Humans, Receptors, Cell Surface, RNA, Messenger, Diagnostic Errors, GPI-Linked Proteins, Polycythemia Vera, Granulocytes
Isoantigens, Membrane Glycoproteins, RNA Stability, Humans, Receptors, Cell Surface, RNA, Messenger, Diagnostic Errors, GPI-Linked Proteins, Polycythemia Vera, Granulocytes
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