[Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells].
Copyright policy )[Analysis of methylation status of the promoter of mdr1 gene in K562 and K562/DNR cells].
To analyze the methylation patterns of mdr1 gene promotor in both K562 cell and K562/DNR cells and the relationship between promotor methylation and P-gp expression.mdr1 gene expression was analyzed by flow cytometry and RT-PCR, methylation status of the promotor of mdr1 gene by bisulfite-sequencing (including two GC-box).mdr1 gene was found methylated at GC-box and not expressed in K562 cells, but unmethylated and expressed respectively in K562/DNR cells.The methylation patterns of mdr1 gene promotor in K562/DNR and K562 cells were different. mdr1 gene silencing was associated with the promotor methylation.
- China Medical University China (People's Republic of)
Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Gene Silencing, Sequence Analysis, DNA, DNA Methylation, Flow Cytometry, K562 Cells, Promoter Regions, Genetic
Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Humans, ATP Binding Cassette Transporter, Subfamily B, Member 1, Gene Silencing, Sequence Analysis, DNA, DNA Methylation, Flow Cytometry, K562 Cells, Promoter Regions, Genetic
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