Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages.
Copyright policy )Oxidized LDL upregulated ATP binding cassette transporter-1 in THP-1 macrophages.
To study the effect of oxidized low density lipoprotein (ox-LDL) on ATP binding cassette transporter A1 (ABCA1) in THP-1 macrophages.After exposing the cultured THP-1 macrophages to ox-LDL for different periods, cholesterol efflux was determined by FJ-2107P type liquid scintillator. ABCA1 mRNA and protein level were determined by reverse trancriptase-polymerase chain reaction (RT-PCR) and Western blot, respectively. The cholesterol level in THP-1 macrophage foam cells was detected by high performance liquid chromatography.ox-LDL elevated ABCA1 in both protein and mRNA levels and increased apolipoprotein (apo) A-I-mediated cholesterol efflux in a time- and dose-dependent manner. 22(R)-hydroxycholesterol and 9-cis-retinoic acid did significantly increase cholesterol efflux in THP-1 macrophage foam cells (P<0.05), respectively. Both of them further promoted cholesterol efflux (P<0.01). As expected, liver X receptor (LXR) agonist decreased content of esterified cholesterol in the macrophage foam cells compared with control, whereas only a slight decrease of free cholesterol was observed. LXR activity was slightly increased by oxidized LDL by 12 % at 12 h compared with 6 h. However, LXR activity was increased about 1.8 times at 24 h, and oxidized LDL further increased LXR activity by about 2.6 times at 48 h.ABCA1 gene expression was markedly increased in cholesterol-loaded cells as a result of activation of LXR/RXR. ABCA1 plays an important role in the homeostasis of cholesterol in the macrophages.
Apolipoprotein A-I, Receptors, Retinoic Acid, Macrophages, Receptors, Cytoplasmic and Nuclear, Orphan Nuclear Receptors, Hydroxycholesterols, DNA-Binding Proteins, Lipoproteins, LDL, Cholesterol, Retinoid X Receptors, Leukemia, Myeloid, Humans, ATP-Binding Cassette Transporters, RNA, Messenger, Oxidation-Reduction, Alitretinoin, ATP Binding Cassette Transporter 1, Foam Cells, Liver X Receptors, Transcription Factors
Apolipoprotein A-I, Receptors, Retinoic Acid, Macrophages, Receptors, Cytoplasmic and Nuclear, Orphan Nuclear Receptors, Hydroxycholesterols, DNA-Binding Proteins, Lipoproteins, LDL, Cholesterol, Retinoid X Receptors, Leukemia, Myeloid, Humans, ATP-Binding Cassette Transporters, RNA, Messenger, Oxidation-Reduction, Alitretinoin, ATP Binding Cassette Transporter 1, Foam Cells, Liver X Receptors, Transcription Factors
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).26 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!