[Designing and clinical testing of immune-enzyme and immunofluorescence test systems for serodiagnosis of ixodes borreliosis].
Copyright policy )[Designing and clinical testing of immune-enzyme and immunofluorescence test systems for serodiagnosis of ixodes borreliosis].
The methods of immune enzyme assay (MIEA) and of lanthanide immunofluorescence analysis (LIFA) were used to work out the test systems for the detection (in blood serum of patients) of specific IgM IgG antibodies to the B. burgdorferi spirochete--a causative agent of ixodic borrelioses. The test system was clinically tested versus the indirect immunofluorescence reaction (IIFR) and commercial immune enzyme test system (CIET). The results of antibodies' detection were shown to correlate with the analysis data for the same sera in IIFR and to be in line with a real presence or absence of the disease. Test systems based on LIFA were proven to be most sensitive and specific.
Immunoenzyme Techniques, Lyme Disease, Immunoglobulin M, Borrelia burgdorferi, Immunoglobulin G, Humans, Serologic Tests, Fluorescent Antibody Technique, Indirect, Antibodies, Bacterial, Sensitivity and Specificity
Immunoenzyme Techniques, Lyme Disease, Immunoglobulin M, Borrelia burgdorferi, Immunoglobulin G, Humans, Serologic Tests, Fluorescent Antibody Technique, Indirect, Antibodies, Bacterial, Sensitivity and Specificity
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