Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

Ole, Vielemeyer; Jill Y, Crouch; Stephen C, Edberg; John G, Howe

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Journal of Clinical Microbiology

Article . 2004

Data sources: Europe PubMed Central

Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

descriptionPublicationkeyboard_double_arrow_right Article 09 Apr 2004 English Journal:Journal of clinical microbiology, volume 42 (issn: 0095-1137,

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Authors: Ole, Vielemeyer; Jill Y, Crouch; Stephen C, Edberg; John G, Howe;

pmid: 14766870

pmc: PMC344463

Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

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Abstract

Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.

Related Organizations

Yale University
United States

Keywords

AIDS-Related Opportunistic Infections, Genotype, Whooping Cough, Restriction Mapping, Sputum, HIV Infections, DNA, Ribosomal, Bordetella pertussis, Rec A Recombinases, RNA, Ribosomal, 16S, Humans

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