Isolation of an immunodiagnostic Taenia solium coproantigen.

M, Maass; E, Delgado; J, Knobloch

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Article . 1993

Data sources: Europe PubMed Central

Isolation of an immunodiagnostic Taenia solium coproantigen.

descriptionPublicationkeyboard_double_arrow_right Article 27 Jan 1993 English Journal:Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ), volume 43 (issn: 0177-2392,

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Authors: M, Maass; E, Delgado; J, Knobloch;

pmid: 1470844

Isolation of an immunodiagnostic Taenia solium coproantigen.

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Abstract

Antigens of Taenia solium can be demonstrated by ELISA technique in stool samples of tapeworm carriers. In order to isolate these immunodiagnostic components from stool, fecal samples of known T. solium carriers were subject to chromatographic purification procedures: immunoaffinity chromatography and a subsequent two-step high performance liquid chromatography yielded a 60-kDa protein that was shown to react in a Western blot with polyclonal anti-T. solium IgG. The protein isolated appeared to be the major antigenic component excreted with the feces of parasite hosts.

Related Organizations

Bernhard Nocht Institute for Tropical Medicine
Germany
Leibniz Association
Germany

Keywords

Taenia, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Chromatography, Affinity, Molecular Weight, Feces, Antigens, Helminth, Immunoglobulin G, Carrier State, Animals, Humans, Electrophoresis, Polyacrylamide Gel, Chromatography, High Pressure Liquid, Taeniasis

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