
Routine fluorescent assays for urine 4-pyridoxic acid (4-PA) in variants of lactone 4-PA external standard (variant 1) and internal 4-PA standard (variant 2) were compared to high performance liquid chromatography. Correlation between the two values were defined by specially derived equation. The internal standard is preferable, as variant 1 presents underestimated results. Variant 2 is limited to high 4-PA concentrations, being unfit in low ones for overestimating the data. The chromatography proved most reliable and specific.
Adult, Spectrometry, Fluorescence, Humans, Female, Middle Aged, Chromatography, High Pressure Liquid, Pyridoxic Acid
Adult, Spectrometry, Fluorescence, Humans, Female, Middle Aged, Chromatography, High Pressure Liquid, Pyridoxic Acid
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