The improvement of monitoring methods of cattle infection with bovine leukemia virus (BLV).
Copyright policy )The improvement of monitoring methods of cattle infection with bovine leukemia virus (BLV).
This study demonstrates the usefulness of PLA and nested-PCR methods for the detection of cattle infection with enzootic bovine leukemia virus. Serological control with PLA was by 1.7% more sensitive than reference ELISA examination. Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR.
Immunodiffusion, Enzyme-Linked Immunosorbent Assay, Enzootic Bovine Leukosis, Antibodies, Viral, Polymerase Chain Reaction, Sensitivity and Specificity, Dairying, Predictive Value of Tests, DNA, Viral, Leukemia Virus, Bovine, Animals, Cattle, Female
Immunodiffusion, Enzyme-Linked Immunosorbent Assay, Enzootic Bovine Leukosis, Antibodies, Viral, Polymerase Chain Reaction, Sensitivity and Specificity, Dairying, Predictive Value of Tests, DNA, Viral, Leukemia Virus, Bovine, Animals, Cattle, Female
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