[Beta-mannosidase of trophoblast and humana skin fibroblasts].
Copyright policy )[Beta-mannosidase of trophoblast and humana skin fibroblasts].
Conditions for assay of beta-mannosidase activity in human chorionic villi were studied using the fluorogenic substrate 4-methylumbelliferyl-beta-D-mannopyranoside. Comparison of the biochemical properties of the chorionic villi beta-mannosidase with those of the enzyme from human cultured fibroblasts showed their similarity. Like the enzyme from skin fibroblasts, the chorionic villi beta-mannosidase had rather high activity. Both enzymes had virtually the same pH optimum (4.2-4.7) and Km value. The data presented suggest that chorion biopsy specimens can be used for prenatal determination of beta-mannosidase activity at the early stage of development.
Biopsy, Prenatal Diagnosis, Mannosidases, beta-Mannosidase, Humans, Fluorometry, Chorionic Villi, Fibroblasts, Hydrogen-Ion Concentration, Cells, Cultured, Skin
Biopsy, Prenatal Diagnosis, Mannosidases, beta-Mannosidase, Humans, Fluorometry, Chorionic Villi, Fibroblasts, Hydrogen-Ion Concentration, Cells, Cultured, Skin
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