The study of impedance line along channel had been performed with the radionuclide tracing method in 15 healthy human being and 4 guinea pigs. These images of tracer migrating routes approximately corresponded with classical courses of channel. The study proved that these line-shaped tracer migration were not by the way of lymphatic and blood vessels as well as nerves. This image of radionuclide migrating along channel may used as the guide for the observation of micro-autoradiography. In this study, satisfactory migrating image along the channel was also obtained by 131I instead of 99mTC. So a new way was opened up for both macro- and micro-autoradiographic study.