Constitutive expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor abrogates all requirements for exogenous growth factors.
Copyright policy )Constitutive expression of insulin-like growth factor 1 and insulin-like growth factor 1 receptor abrogates all requirements for exogenous growth factors.
BALB/c3T3 cells are exquisitely growth regulated and require both platelet-derived growth factor and insulin-like growth factor-1 (IGF-1) for optimal proliferation. BALB/c3T3 cells that constitutively express IGF-1 and elevated levels of IGF-1 receptor (IGF-1R) are capable of growth in serum-free medium without the addition of any exogenous growth factors. BALB/c3T3 cells overexpressing only the IGF-1R plasmid required IGF-1 or insulin for serum-free growth. Antisense oligodeoxynucleotides complementary to IGF-1R mRNA inhibited IGF-1-mediated cell growth. Under these conditions, neither the epidermal growth factor receptor nor phospholipase C gamma 1 was autophosphorylated. These findings indicate that constitutive expression of IGF-1 and IGF-1R allows 3T3 cells to grow in serum-free medium without addition of those exogenous growth factors that are required by the parent cell line.
Platelet-Derived Growth Factor, Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Receptors, Cell Surface, Receptors, Somatomedin, 3T3 Cells, DNA, Transfection, Culture Media, Serum-Free, ErbB Receptors, Mice, Animals, RNA, Antisense, RNA, Messenger, Insulin-Like Growth Factor I, Phosphorylation, Protein Processing, Post-Translational, Cell Division
Platelet-Derived Growth Factor, Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Receptors, Cell Surface, Receptors, Somatomedin, 3T3 Cells, DNA, Transfection, Culture Media, Serum-Free, ErbB Receptors, Mice, Animals, RNA, Antisense, RNA, Messenger, Insulin-Like Growth Factor I, Phosphorylation, Protein Processing, Post-Translational, Cell Division
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).190 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!