This investigation was aimed at the study of distribution, localization and morphological peculiarities of NO-ergic cells and fibers in the digestive tract of Japanese anchovy Engraulis japonicus. Using the histochemical method developed by B.T. Hope and S.R. Vincent (1989), NO-ergic cells and fibers were demonstrated in the intermuscular plexus and in the circular layer of the muscular tunic in all the digestive tract organs studied. Single No-ergic cells were located in the longitudinal layer of the muscular tunic in the esophagus and stomach. In the submucosal plexus NO-ergic neurons were detected only in the stomach. The greatest density of NO-ergic cell distribution was characteristic to the posterior portion of the intestine and stomach.