The purpose of the work was to appraise the correlation between magnitude of iodine excretion with urine and concentration of iodine ions in drinking water in the population living in the Lublin province. The tests were also performed among the citizens of the Zamość voivodship. Mean concentration of iodine in urine of the tested citizens was 132.0 micrograms/g creatinine. Mean value of I ions in drinking water was 27.5 micrograms/l. The obtained results of concentration of iodine both in urine and drinking water did not differ as shown in references. Statistically significant correlation between the measure of I excretion in urine and I ions concentration in drinking water was confirmed. There is no ascertained deficiency of iodine in Zamość region inhabitants. The concentration of iodine in drinking water from adjacent wells was fluctuating in wide limits, but did not differ from recommended norms. There is a positive correlation between concentration of iodine in drinking water and its level in excreted urine.