Defining the SNARE complex binding surface of alpha-SNAP: implications for SNARE complex disassembly.
Copyright policy )Defining the SNARE complex binding surface of alpha-SNAP: implications for SNARE complex disassembly.
N-Ethylmaleimide-sensitive factor (NSF) and its adaptor protein alpha-soluble NSF attachment protein (alpha-SNAP) sustain membrane trafficking by disassembling soluble NSF attachment protein receptor (SNARE) complexes that form during membrane fusion. To better understand the role of alpha-SNAP in this process, we used site-directed mutagenesis to identify residues in alpha-SNAP that interact with SNARE complexes. We find that mutations in charged residues distributed over a concave surface formed by the N-terminal nine alpha-helices of alpha-SNAP affect its ability to bind synaptic SNARE complex and promote its disassembly by NSF. Replacing basic residues on this surface with alanines reduced SNARE complex binding and disassembly, whereas replacing acidic residues with alanines enhanced alpha-SNAP efficacy in both assays. These findings show that the ability of NSF to take apart SNARE complexes depends upon electrostatic interactions between alpha-SNAP and the acidic surface of the SNARE complex and provide insight into how NSF and alpha-SNAP work together to drive disassembly.
- University of Mary United States
Models, Molecular, Sequence Homology, Amino Acid, Macromolecular Substances, Molecular Sequence Data, Static Electricity, Vesicular Transport Proteins, Membrane Proteins, In Vitro Techniques, Membrane Fusion, Recombinant Proteins, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins, Kinetics, Amino Acid Substitution, Mutagenesis, Site-Directed, Animals, Cattle, Amino Acid Sequence, Carrier Proteins, SNARE Proteins, N-Ethylmaleimide-Sensitive Proteins
Models, Molecular, Sequence Homology, Amino Acid, Macromolecular Substances, Molecular Sequence Data, Static Electricity, Vesicular Transport Proteins, Membrane Proteins, In Vitro Techniques, Membrane Fusion, Recombinant Proteins, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins, Kinetics, Amino Acid Substitution, Mutagenesis, Site-Directed, Animals, Cattle, Amino Acid Sequence, Carrier Proteins, SNARE Proteins, N-Ethylmaleimide-Sensitive Proteins
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