To study the relationship between the overexpression of CYFRA21-1 in the serum of laryngeal cracinoma patients and the associated clinical feature.The serum level of CYFRA21-1 were determined by ELISA in 25 laryngeal carcinoma patients preoperatively and postoperatively. 20 laryngeal benign diseases are as control group.Positive rate was 60% for serum of CYFRA21-1 in the laryngeal carcinoma patiens. The mean serum level for CYFRA21-1 in laryngeal carcinoma patients preoperatively (5.14 +/- 1.82) micrograms/L was significantly elevated than in control group (2.17 +/- 0.68 micrograms/L). The Serum level of CYFRA21-1 1 were significantly declined after surgery under normal level. Serum level of CYFRA21-1 went up once ogain in 2 recurrence patients. There was no correlation between serum level of CYFRA21-1 with age, sex and site in the laryngeal carcinoma patients. There were correlations between the serum level of CYFRA21-1 with clinical stage, pathological grading and lymphatic matatasis. Level of CYFRA21-1 was higher in poorly differentiated.The study suggests that serem CYFRA21-1 level could be a useful tumor marker for diagnosis, progenosis and follow-up after surgery in laryngeal carcinomas.