[Cloning and expression of caiDE genes in E. coli BL21(DE3)].
Copyright policy )[Cloning and expression of caiDE genes in E. coli BL21(DE3)].
The caiDE genes for carnitine racemase and its relative factor were cloned into pUC18 from the chromosomal DNA of E. coli MC4100 by shot-gun approach and have been verified by sequence analysis. The target plasmid was named as pJX393 and subcloned to form a transcription plasmid pDSW2. After its transformation into BL21(DE3) strain and induction by IPTG, the recombinant strain containing pDSW2 expressed two apparent protein bands on SDS-PAGE, whose molecular weight were 30 kD and 24 kD individually.
DNA, Bacterial, Base Sequence, Escherichia coli Proteins, Molecular Sequence Data, Escherichia coli, Racemases and Epimerases, Gene Expression, Transformation, Bacterial, Cloning, Molecular, Recombinant Proteins, Plasmids
DNA, Bacterial, Base Sequence, Escherichia coli Proteins, Molecular Sequence Data, Escherichia coli, Racemases and Epimerases, Gene Expression, Transformation, Bacterial, Cloning, Molecular, Recombinant Proteins, Plasmids
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