[Cloning and sequencing of the Chinese bovine prion protein (PrPC) gene].

D, Wang; X, Wang; S, Han; B, Tian; Z, Rao

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ACTA MICROBIOLOGICA SINICA

Article . 2003

Data sources: Europe PubMed Central

[Cloning and sequencing of the Chinese bovine prion protein (PrPC) gene].

descriptionPublicationkeyboard_double_arrow_right Article 21 Nov 2003Journal:Wei sheng wu xue bao = Acta microbiologica Sinica, volume 38 (issn: 0001-6209,

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Authors: D, Wang; X, Wang; S, Han; B, Tian; Z, Rao;

pmid: 12548919

[Cloning and sequencing of the Chinese bovine prion protein (PrPC) gene].

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Abstract

The total DNA was isolated from lymphocyte of peripheral blood of Chinese cattle. The PrPC gene was amplified by polymerase chain reaction, using two primers. It was then cloned into pGEM-T Easy Vector. The result of DNA sequencing was indicated that the 795 bp amplified fragment contains the entire PrPC coding sequence, which has no intron and is same as the published gene sequence.

Related Organizations

Institute of Microbiology
China (People's Republic of)
Chinese Academy of Sciences
China (People's Republic of)

Keywords

Base Sequence, Prions, Molecular Sequence Data, Animals, Cattle, Amino Acid Sequence, Cloning, Molecular, Polymerase Chain Reaction, DNA Primers

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