Carbohydrate has many important functions in various biologic processes. Capillary electrophoresis (CE) is one of the key tools of carbohydrate analysis. Chip CE is a new but efficient technique in the study of life science. Carbohydrate analysis with CE and Chip CE are reviewed. Separation strategies and detection of CE for various carbohydrates, including monosaccharides, polysaccharides and glycoconjugates are described. The application and potential ability of Chip CE in carbohydrate research are reviewed as well.