To investigate the effect and distribution of cholinergic efferent vestibular neurons in normal wistar rat.HPR retrograde tracer and Immunocytochemical techniques were used.Results show that AchT immunoreactive neurons locate in dorsolateral and dorsomedial to genu of the facial. Few neurons scatter in the parvocellular nucleus.As a neural transmitter, acetylcholine distribute in efferent vestibular neurons and play an important role.