To evaluate clinical effects of iodophors on root canal therapy.147 teeth with root canal therapy collected from clinical patients were divided into two groups: experimental and control group. In experimental group, 0.05% and 0.5% iodophors were taken as irrigate agent and disinfectant, respectively, while in control group, 3% H2O2 + 0.9% NS and Fc were used.Clinical therapeutic effects evaluation indicated that there was no statistically significant difference of alteration of clinical syndromes after the administration and the therapeutic effect between experimental group and control group during the period of root canal therapy.Iodophors could be taken as a kind of candidate drugs on irrigation and disinfection of root canal therapy.